Toshio murashige biography channel wikipedia

Murashige and Skoog medium or MSO or MS0 MS-zero is the most popular plant growth medium used in the laboratories worldwide for cultivation of plant cell culture on agar. Skoog in during Murashige's search for a new plant growth regulator.

Toshio Murashige.

A number behind the letters MS is used to indicate the sucrose concentration of the medium. Along with its modifications, it is the most commonly used medium in plant tissue culture experiments in the laboratory. As Skoog's doctoral student, Murashige originally set out to find an as-yet undiscovered growth hormone present in tobacco juice. No such component was discovered; instead, analysis of juiced tobacco and ashed tobacco revealed higher concentrations of specific minerals in plant tissues than were previously known.

A series of experiments demonstrated that varying the levels of these nutrients enhanced growth substantially over existing formulations. It was determined that nitrogen in particular enhanced growth of tobacco in tissue culture.

Toshio Murashige.

According to recent scientific findings, however, MS medium is not suitable as a nutrient solution for deep water culture or hydroponics. Contents move to sidebar hide. Article Talk. Read Edit View history. Tools Tools. Download as PDF Printable version. In other projects. Wikimedia Commons Wikidata item. Growth medium used in plant cell culture.